DBA Porosity Analysis of Human Bones by Double-Beam Acoustography
DOI:
https://doi.org/10.29384/rbfm.2026.v20.19849001871Keywords:
double-beam acoustography, human trabecular bones, photoacoustic microscopyAbstract
Dual-beam acustography is an elastography technique that uses focused ultrasonic radiation to extract mechanical information through spectral analysis of the acoustic signal generated by the interaction of the ultrasonic pulse with the medium's structure. This study focuses on applying dual-beam acustography to analyze the porosity of human bone. The project involves measuring and analyzing trabecular bone samples from human vertebrae, with ethical approval. These samples, previously tested for mechanical properties at the Laboratory of Manufacturing and Precision Engineering at the School of Engineering of São Carlos, were prepared using a 3D-printed support and analyzed in a water tank with an ultrasonic transducer and hydrophone. Additionally, photoacoustic microscopy was incorporated to provide a complementary perspective, enhancing the understanding of bone porosity. The comparison between techniques revealed similar signals for assessing bone porosity. The acoustic signal showed moderate correlation with the elastic modulus, suggesting that samples with higher elastic modulus exhibit higher signals. The ultimate tensile strength (TLRF) demonstrated a strong correlation with the signal, while the ultimate strain (DLRF) showed a weak correlation. These results indicate that TLRF is a good indicator of bone integrity, whereas DLRF may not be suitable for general evaluations.
Downloads
References
1. Jee WSS. Bone histomorphometry: techniques and interpretations. Boca Raton: CRC Press; 2001.
2. Lucchinetti E, Thomann D, Danuser G. Micromechanical testing of bone trabeculae - potentials and limitations. J Mater Sci. 2000;35:6057–65. https://doi.org/10.1023/A:1026748913553
3. Lips P, Courpron P, Meunier PJ. Mean wall thickness of trabecular bone packets in the human iliac crest: changes with age. Calcif Tissue Res. 1978;26(1):13–7.
4. Oftadeh R, Perez-Viloria M, Villa-Camacho JC, Vaziri A, Nazarian A. Biomechanics and mechanobiology of trabecular bone: a review. J Biomech Eng. 2015;137(1):0108021–15. doi: 10.1115/1.4029176.
5. Cai X, Zhang YS, Xia Y, Wang LV. Photoacoustic microscopy in tissue engineering. Mater Today. 2013;16(3):67–77. https://doi.org/10.1016/j.mattod.2013.03.007
6. Silva GT, Mitri FG. Difference-frequency generation in vibro-acoustography. Phys Med Biol. 2011;56(18):5985–93. Available from: https://iopscience.iop.org/article/10.1088/0031-9155/56/18/013/pdf
7. Braz GA. Implementação de uma técnica de caracterização de tecidos biológicos baseada na emissão acústica em baixa frequência (kHz) de alvos excitados em alta frequência (MHz) [dissertação]. São Paulo: Universidade de São Paulo; 2020. Available from: https://www.teses.usp.br/teses/disponiveis/59/59135/tde-31072020-162346/publico/Corrigida_GuilhermedeAraujoBraz.pdf
8. Uliana JH, Miano ACC, Silva GT, Mitri FG, Baffa O. Photoacoustic microscopy of sandstone reservoirs rocks. In: 2023 IEEE International Ultrasonics Symposium (IUS); 2023 Sep; Montreal, Canada. Piscataway: IEEE; 2023. https://doi.org/10.1109/IUS51837.2023.10308378
9. Uliana JH. Sistemas de imagem e espectroscopia fotoacústica [tese]. Ribeirão Preto: Universidade de São Paulo; 2021. Available from: https://www.teses.usp.br/teses/disponiveis/59/59135/tde-10102022-092326/
10. Keaveny TM, Morgan EF, Niebur GL, Yeh OC. Biomechanics of trabecular bone. Annu Rev Biomed Eng. 2001;3:307–33. doi: 10.1146/annurev.bioeng.3.1.307.
11. Cezar R. Caracterização de materiais por técnicas não destrutivas [tese]. São Carlos: Escola de Engenharia de São Carlos, Universidade de São Paulo; 2015. Available from: https://teses.usp.br/teses/disponiveis/18/18150/tde-09022015-113119/publico/Tese_doutorado_Reinaldo_Cesar.pdf
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Dorival Mantovani, Frederico Possato Tagliaferro, João Henrique Uliana, Reinaldo Cesar, Theo Zeferino Pavan, Antonio Adilton Oliveira Carneiro
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The submission of original articles to the Brazilian Journal of Medical Physics implies the transfer, by the authors, of the rights of print and digital publication. Copyright for published articles remains with the author, with journal rights on first publication. Authors may only use the same results in other publications by clearly indicating this journal as the original publisher. As we are an open access journal, free use of articles in educational, scientific, non-commercial applications is allowed, as long as the source is cited.
The Brazilian Journal of Medical Physics is under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0).
